The latest Krystal™ Glass Bottom plates combine the advantageous optical properties of the industry-leading cover glass technology with the versatility of a microplate. These glass bottom plates offer high optical quality, thermal stability, chemical resistance, and low autofluorescence to suit a wide range of samples for accurate and reliable light and fluorescence readings.
The Krystal™ glass bottom plate range demonstrate higher performance than standard polystyrene plates for fluorescence assays, luminescence detection, scintillation counting and high-resolution microscopy using confocal imaging.
Special thickness of 170 µm tolerance of +/- 5 µm is also available for high sensitivity applications.
The Krystal™ 384-well microplate range incorporates novel square wells with a large liquid capacity of 120 µl per well, increasing the achievable sensitivity of most HTS assays.
Using a proprietary manufacturing technique, the Krystal 384 offers market leading plate flatness (+/- 0.1mm tolerance) that translates into a significant increase in measurement precision and elimination of read errors when performing cell based assays using fluorescent or luminescent imaging. All tissue-culture treated plates are supplied lidded and sterile, in individual bags.
Manufactured for whole-plate CCD imaging and laser detection applications, Krystal glass bottom plates consist of a polystyrene upper part and a clear borosilicate glass sheet fixed to the base with a proprietary adhesive. This process results in consistent flatness of the base and gives improved light transmission whilst maintaining a flat optical plane for growing cells. The nominal cut-off wavelength of 335nm allows most fluorescence assays to be excited or read through the glass bottom. All plates are supplied lidded.
Recently, scientists have begun using assay chemistries which require excitation or detection wavelengths in the far UV region, below 350nm. To enable our customers to develop assays in this area, Porvair Sciences has introduced a very high specification range of COP-bottomed, UV-transparent microplates. Precision engineered and assembled, these plates are available in 96- and 384-well black matrix only for the most sensitive UV range fluorescence assays using whole-plate imaging or confocal microscopy.